Проекти

KP06-Russia/19/28.09.2019 „Multivariative Raman and fluorescence diagnosis of cutaneous tumors“

Funded by NSF-MES-Bulgaria

Partners:

Institute of Electronics, Bulgarian Academy of Sciences

Samara National Research University, Department of Laser and Biotechnical Systems

Bulgarian scientific team

Alexander Gisbrecht, Assoc. prof., PhD – team coordinatorEkaterina Borisova, Assoc. prof., PhDPetranka Troyanova, Prof., PhD, MD in DermatologyPetya Pavlova, Assoc. prof., PhDTsanislava Genova, PhD studentVictoria Mircheva, BS student in “Medical physics”, SU, technician – IE-BASStoyan Ilyov, BS student in “Medical physics”, SU, technician – IE-BAS

Russian scientific team

Valeriy Zakharov, Prof., D. Sci. – team coordinatorIvan Bratchenko, Assoc. prof., PhD in OpticsAlexander Moryatov, Assoc. prof., MD in OncologySergey Kozlov, Prof., D Sci in OncologyOleg Myakinin, Assist., MSDmitry Raulov, PhD studentLydmila Shamina, PhD student, Laser systemsDmitrii Artemyev, assistant, engineer, MSJulia Christoforova, PhD student

PROJECT OBJECTIVES

Broadening and fruitful development of scientific research collaboration between Institute of Electronics – BAS, laboratory “Biophotonics”, Bulgaria and Samara National Research University “Acad. S.P. Korolev”, Department of Laser and Biotechnical Systems, Russian Federation, in the field of autofluorescence, diffuse reflectance and Raman scattering spectroscopy and topography, as well multispectral analysis of skin tumors.
Expanding of opportunities of existing in both organizations experimental spectroscopic practices and increasing of the collected databases for fluorescence and scattering (Raman) properties of skin pathologies, by means of multispectral analysis of spectral data and images, to obtain new knowledge and to achieve optimization of diagnostic algorithms with clinical applicability. Training of young scientists and an exchange of best practices between the partners for the spectroscopic techniques development and application for the skin pathologies in vivo analysis.
Experimental studies on the development and optimization of optoelectronic instrumentation, discrimination algorithms and methodology of multispectral analysis and diagnostics of normal and pathological skin tissues, their approbation and implementation in clinical practice for early diagnosis of skin malignancies. Optimization of the applied spectroscopic techniques to obtain maximum diagnostic accuracy in the determination of the principal non-melanoma skin cancers and malignant melanoma vs. other pigmented and dysplastic cutaneous conditions.

USED METHODS

The methods of autofluorescence and Raman scattering for spectral analysis ex vivo and in vivo will be used to determine the diagnostically significant spectral characteristics in benign, dysplastic and malignant skin pathologies. Methods of diffuse reflectance spectroscopy and colorimetry will be used for broadening of diagnostic capabilities and will be applied to pigmented lesions, including malignant melanoma. Russian researchers will apply developed by themselves algorithms for multivariate spectral analysis of images and will up-grade the possibilities of the Bulgarian team for analysis and differentiation of cutaneous pathologies. Measurements will be made in two modes –with a fiber optic probe – one-dimensional spot measurements (1-D), and topographic (2-D) images with CCD cameras with high spectral sensitivity of the distribution of autofluorescence, scattering and diffuse-reflectance signal from the normal and pathologic tissues independently for each partner and in the joint experimental sessions during research visits.
The spectral techniques for autofluorescence and Raman scattering analysis will be integrated in a common system of multispectral analysis of the tissues, to obtain original experimental data on spectral properties of the tested cutaneous lesions, with subsequent development of algorithms of diagnostics and differentiation of skin tumors.
Autofluorescence, Raman, reflectance and colorimetric properties combined databases will be created and calculation algorithms will be introduced – development of diagnostic algorithms for differentiation of the major types of malignant neoplasia by type, stage of development and vs. benign lesions. Spectral data and diagnostics definitions will be compared and verified with getting ”gold standard” histological results.
Recently established cooperation between two research groups lead to exchange of knowledge and ideas about optimization of spectroscopic investigations of cutaneous neoplasia carried out. They were presented in three collaborative research papers, published in the period 2017-2019, which is a significant indicator for the actuality and importance of the planned bilateral investigations.

графика

NIR-AF EEM maps ex vivo of different skin lesions at excitation from 635 to 785 nm with step 10 nm: (a) normal skin, (b) benign nevus, (c) dysplastic nevus, and (d) malignant melanoma.

EXPECTED RESULTS

Research contacts will be established and expanded between two partner organizations and up – grade of the knowledge and technical skills of both teams scientists will be achieved in the field of multivariate fluorescent and Raman spectral analysis of cutaneous pathologies. New and original experimental results for the spectral properties of different types of benign, dysplastic and malignant skin neoplasia will be produced and correlated with the spectral characteristics – type and stage of development, for the needs of their diagnostic and prognostic applications in clinical onco-dermatology. Results allowing an increase of the diagnostic accuracy are expected to be achieved, noninvasive and in real time.
Autofluorescence, diffuse-reflectance and colorimetric diagnostics technique for skin tumours detection, created in some previous studies, will be improved from the Bulgarian side. New knowledge and data for Raman scattering properties of cutaneous neoplasia will be received. New database in 2-D regime of detection will be collected, which allows to receive spectral images of skin tumours. Hardware and methodological tools will be implemented clinically. Russian team will achieve optimization of their method for multivariative spectral analysis of skin in vivo, based on the spectral measurements of Bulgarian team in regime of autofluorescence and diffuse-reflectance, combined wirh the data of Raman spectroscopy of skin neoplasia.
Research results will be published in specialized international journals and conference proceedings, necessary for their promotion and will contribute to the overall research growth of the participants, members of the project team. Developed methodologies and algorithms will be used in the dissertation of a one PhD student for each partner; as well two Bachelor degree theses will be prepared from Bulgarian side for two students. It is foreseen a Master degree thesis preparation, based on the project results. In this way the conducted research and established cooperation will be prerequisites for a start of a PhD studentship for one of the Bulgarian team members, which will allow being achieved a sustainable development of the IE- BAS human resources in the biophotonics research field.

Presentation under project #KP06-Russia/19/28.09.2019 „Multivariative Raman and fluorescence diagnosis of cutaneous tumors“:

    1. Ильов, С., Генова, Ц., Иванов, Д., Троянова, П., Братченко, И., Лихачовс, А., Спигулис, Я., Борисова, Е., Спектрални характеристики на меланин-пигментирани кожни новообразувания, VIII Национална студентска научна конференция по физика и инженерни технологии с международно участие, 31.10.2019 – 01.11.2019, Пловдив, България (Poster presentations);
    2. Borisova E., Оптическая спектроскопия биологических тканей как инструмент для диагностики первичного рака и мониторинга лечения, XVII Всероссийский молодежный Самарский конкурс-конференция научных работ по оптике и лазерной физике, 12.11.2019 – 16.11.2019, Самара, Русия (Plenary presentations);
    3. Zakharov V., Bratchenko L., Khristoforova Yu., Myakinin O., Artemyev D., Moryatov A., Kozlov S., Borisova E., Genova Ts., Troyanova P., Bratchenko I., Multiparametric spectral diagnosis of skin cancer, Photonics Europe 2020 – Conference “Tissue Optics and Photonics”, 29.03.2020 – 03.04.2020, Strassbourg, France (Invited presentation).
    4. Khristoforova Yu., Bratchenko I., Borisova E., Bratchenko L., Genova Ts., Gisbreht Al., Moryatov A., Kozlov S., Troyanova P., Zakharov V., The study of ex vivo and in vivo melanocytic skin neoplasms using near-infared fluorescence spectroscopy, VI International Conference on Information Technology anf Nanotechnology, 26.05.2020 – 29.05.2020, Самара, Русия (Устен доклад) ЛИНК
    5. Bratchenko L., Abrosimova E., Stafeev S., Tupikova E., Borisova E., Bratchenko I., “Conventional Raman and Surface-Enhanced Raman Spectroscopy for Human Skin Components Analysis” VI International Conference on Information Technology anf Nanotechnology, 26.05.2020 – 29.05.2020, Самара, Русия (Poster presentation);
    6. Khristoforova Y., Bratchenko I., Bratchenko L., Myakinin O., Artemtev D., Zakharov V., Moryatov A., Kozlov S., Borisova E., Genova Ts., Troyanova P.., „Optical biopsy of skin cancer based on Raman and fluorescence spectroscopy“, 4th International Conference Terahertz and Microwave Radiation: Generation, Detection and Applications, 24.08.2020 – 26.08.2020, Tomsk, Russian Federation (Oral presentations);
    7. Borisova E., Genova Ts., Troyanova P., Bratchenko I., Bratchenko L., Zakharov V., Lihacova I., Lihacovs A., Spigulis J..“Multispectral fluorescence detection and imaging of skin tumours, 3rd International Confenrece “Biophotonics-Riga”, 24.08.2020 – 26.08.2020, Riga, Latvia (Invited presentation)
    8. Mircheva V., Zaharieva L., Ilyov S., Troyanova P., Lihacova I., Lihacovs A., Bratchenko I., Bratchenko L., Khristoforova Yu., Zakharov V., Avramov L., Borisova E.. “near-infrared autofluorescence spectroscopy and photobleaching detection of human cutaneous tissues -tool for melanin pigmented skin cancer detection”, 21st International Conference and School on Quantum Electronics “Laser Physics and Applications”, 21.09.2020 – 24.09.2020, Sofia, Bulgaria (virtual forum) (Poster presentation);
    9. Borisova E., Genova Ts., Mircheva V., Ilyov S., Zaharieva L., Ivanov D., Gisbrecht A., Avramov L., Lihachova I., Lihachovs A., Spigulis J., Bratchenko I., Myakinin O., Zakharov V., Terziev I., Troyanova P.., “Skin optical spectroscopy – diagnostics of cancerous and degenerative diseases”, 19th International Conference Laser Optics, 02.11.2020 – 06.11.2020, Sankt Petersburg, Russian Federation (virtual forum) (Poster presentation);

Publications under project #KP06-Russia/19/28.09.2019 „Multivariative Raman and fluorescence diagnosis of cutaneous tumors“:

    1. Borisova E., Bratchenko I., Khristoforova Y., Bratchenko L., Genova Ts., Gisbrecht A., Moryatov A., Kozlov S., Troyanova P., Zakharov V.. Near-infrared autofluorescence spectroscopy of pigmented benign and malignant skin lesions. Optical Engineering, 59, 6, SPIE, 2020, ISSN:0091-3286, DOI:10.1117/1.OE.59.6.061616, 061616-061616-10, JCR-IF(WoS):1.209 Q1 (Scopus) ЛИНК;
    2. Zakharov V., Bratchenko L., Khristoforova Yu., Myakinin O., Artamiev D., Moryatov A., Kozlov S., Borisova E., Genova Ts., Troyanova P., Bratchenko I.. Multiparametric spectral diagnosis of skin cancer. Proceedings SPIE, 11363, 1136310 -1-1136310 -6, 2020, ISSN:0277-786X, DOI:https://doi.org/10.1117/12.2555306, SJR (Scopus):0.24 ЛИНК;
  1. Bashkatov A., Zakharov V., Bucharskaya A., Borisova E., Khristoforova Yu., Genina E., Tuchin V.. Chapter 1: Malignant Tissue Optical Properties. Multimodal Optical Diagnostics of Cancer, Springer Nature Switzerland AG, 2020, ISBN:978-3-030-44594-2, DOI:10.1007/978-3-030-44594-2_1, 103, 3-106 ЛИНК;
  2. Zakharov V., Bratchenko L., Khristoforova Yu., Myakinin O., Artamiev D., Moryatov A., Kozlov S., Borisova E., Genova Ts., Troyanova P., Bratchenko I.. Multiparametric spectral diagnosis of skin cancer. Proceedings SPIE, 11363, SPIE, 2020, ISSN:0277-786X, 1136310, SJR=0.24 ЛИНК;
  3. Христофорова Ю., Братченко И., Борисова Е., Братченко Л., Генова Ц., Гизбрехт А., Морятов А., Козлов С., Троянова П., Захаров В.. ИССЛЕДОВАНИЕ EX VIVO И IN VIVO МЕЛАНОЦИТАРНЫХ НОВООБРАЗОВАНИЙ КОЖИ С ПОМОЩЬЮ ФЛУОРЕСЦЕНТНОЙ СПЕКТРОСКОПИИ В БЛИЖНЕЙ ИК ОБЛАСТИ. ИНФОРМАЦИОННЫЕ ТЕХНОЛОГИИ И НАНОТЕХНОЛОГИИ (ИТНТ-2020) Сборник трудов по материалам VI Международной конференции и молодежной школы. В 4-х томах. Под редакцией С.В. Карпеева . 2020, Самарский национальный исследовательский университет имени академика С.П. Королева (Самара), 2020, 565-572 (AIS eLibrary) ЛИНК;
  4. Братченко Л., Абросимова Е., Стафеев С., Тупикова Е., Борисова Е., Братченко И.. РАМАНОВСКАЯ СПЕКТРОСКОПИЯ И ПОВЕРХНОСТНО-УСИЛЕННАЯ РАМАНОВСКАЯ СПЕКТРОСКОПИЯ ДЛЯ АНАЛИЗА КОМПОНЕНТОВ КОЖИ ЧЕЛОВЕКА. ИНФОРМАЦИОННЫЕ ТЕХНОЛОГИИ И НАНОТЕХНОЛОГИИ (ИТНТ-2020) Сборник трудов по материалам VI Международной конференции и молодежной школы. В 4-х томах. Под редакцией С.В. Карпеева . 2020, Самарский национальный исследовательский университет имени академика С.П. Королева (Самара), 2020, 504-509 (AIS eLibrary) ЛИНК;
  5. Borisova E., Genova Ts., Mircheva V., Troyanova P., Bratchenko I., Bratchenko L., Khristoforova Yu., Zakharov V., Lihacova I., Lihacovs A., Spigulis J.. Multispectral Fluorescence Detection of Pigmented Cutaneous Tumours. Proceedings SPIE, 11585, SPIE, 2020, DOI:10.1117/12.2581967, 1158504. SJR=0.22 ЛИНК;
  6. Ilyov, S., Genova, C., Ivanov, D., Troyanova, P., Bratchenko, I., Bratchenko, L., Zakharov, V., Lihachevs, A., Lihachova, I., Spigulis, J., Borisova, E.. Spectral Characteristics of Melanin-Pigmented Cutaneous Neoplasia. Journal of Physics and Technology, 4, 1, Plovdiv University Press “Paisii Hilendarski”, 2020, ISSN:2535-0536, 41-44 ЛИНК;
  7. Bratchenko L., Abrosimova E., Stafeev S., Tupikova E., Borisova E., Bratchenko I.. Conventional Raman and surface-enhanced Raman spectroscopy for human skin components analysis. IEEE Xplore, IEEE, 2020, DOI:10.1109/ITNT49337.2020.9253245, 1-3 ЛИНК;
  8. Khristoforova Y., Bratchenko I., Borisova E., Bratchenko L., Moryatov A., Kozlov S., Troyanova P., Zakharov V.. The study of ex vivo and in vivo skin neoplasms using near-infrared fluorescence spectroscopy. IEEE Xplore, IEEE, 2020, DOI:10.1109/ITNT49337.2020.9253247., 1-4 ЛИНК;
  9. Khristoforova Yu., Bratchenko I., Bratchenko L., Myakinin O., Artemyev D., Moryatov A., Kozlov S., Borisova E., Genova Ts., Troyanova P., Zakharov V.. Optical biopsy of skin cancer based on Raman and fluorescence spectroscopy. SPIE Proceedings, 11582, SPIE, 2020, 115821K. SJR=0.24 ЛИНК;
  10. Khristoforova Yu., Bratchenko I., Bratchenko L., Myakinin O., Artemyev D., Moryatov A., Kozlov S., Borisova E., Genova Ts., Troyanova P., Zakharov V.. Optical biopsy of skin cancer based on Raman and fluorescence spectroscopy. SPIE Proceedings, 11582, SPIE, 2020, 115821K. SJR =0.24 ЛИНК;